丙型肝炎病毒在細胞培養中的完全復制

讓締一生物跟你們一起解密丙型肝炎病毒在細胞培養中的完全復制吧！

在丙型肝炎病毒（丙型肝炎病毒）生命周期的許多方面還沒有被復制在細胞培養，這已經減緩了這一重要的人類病原體的研究進展。在這里，我們描述了一個全長HCV基因組復制并產生細胞培養中的病毒顆粒（病毒）。復制HCVcc是強大的，在48小時內生產近105感染單位每毫升。病毒顆粒的過濾、中和與病毒糖蛋白E2單克隆抗體。病毒進入依賴于一個假定的HCV受體細胞表達CD81。病毒的復制是由干擾素α的HCV特異性抗病毒化合物的抑制，這表明在體外系統將有助于提高抗病毒藥物的搜索。（友情提示：了解細胞培養基，請點擊這里）

英文原文：

Complete Replication of Hepatitis C Virus in Cell Culture

Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 105 infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-α and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.

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