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產(chǎn)品目錄
  • 細(xì)胞培養(yǎng)進(jìn)口血清
    進(jìn)口胎牛血清
    進(jìn)口新生牛血清
    進(jìn)口豬血清
    馬血清
  • 支原體檢測盒及標(biāo)準(zhǔn)品
    常規(guī)PCR檢測試劑盒
    熒光定量PCR檢測(qPCR法)
    支原體DNA提取
    靈敏度標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    特異性標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    PCR定量標(biāo)準(zhǔn)品(可用于方法驗(yàn)證)
  • 支原體祛除試劑
    細(xì)胞中支原體祛除
    環(huán)境支原體祛除
    水槽支原體祛除
  • 干細(xì)胞培養(yǎng)基
  • DNA/RNA污染祛除
    DNA/RNA污染祛除試劑
    DNA污染監(jiān)測
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    RNA病毒檢測試劑盒
    病毒RNA提取
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    DNA污染監(jiān)測祛除
    PCR/qPCR儀性能檢查
    PCR試劑
    PCR試劑盒
    PCR預(yù)混液(凍干粉)
    熱啟動聚合酶MB Taq DNA
  • 微生物PCR檢測
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預(yù)防和清除支原體污染的細(xì)胞培養(yǎng)方法

2016-10-09 12:47
文章附圖

Since one of the main sources of mycoplasma is the cell cultures brought from outside, it is suggested to supply cells from reliable cell banks.

In the case of the existence of mycoplasma contaminated cell culture in quarantine and the absence of separate incubators, only flasks in a plastic box with lid should be used. Never use plates and unsealed dishes in quarantine. In the case of suspected cultures, handling them at the end of the workday after all other cell culture work is completed, using separated media and reagents, and finally disinfecting the laminar flow hood after working is strongly suggested .

Only use antibiotics responsibly

Although ideally antibiotics used for cell culture should eradicate all contaminants, be nontoxic for the host cells and not interfere with experiments, none of the available antibiotics meet the mentioned criteria. Therefore, application of antibiotics in cell culture should be limited. Instead, a good aseptic practice plays an important role in prevention of contamination.

Microbial contamination in cell culture which is antibiotic free is detectable by turbidity or color changes in cell culture medium. In the case of using antibiotics in a cell culture, there are four possibilities: 1. susceptibility to antibiotics, 2. resistance to antibiotics, 3. partial resistance to antibiotics, 4. resistance to antibiotics only by mycoplasma. The last one is the worst contamination since mycoplasmas can be spread by aerosols. In the case of antibiotic susceptibility, antibiotics prevent the cultivation of bacteria and fungi, but are incapable of precluding mycoplasma from the beginning. Therefore, continuous use of antibiotics for a long time in cell cultures not only is not helpful, but also can cause more problems. However, the use of antibiotics (Penicillin/ Streptomycin) for a short term (the first two weeks) in primary culture is vital. Since antibiotics are unstable in the medium, it is highly suggested to replace antibiotic containing medium with fresh medium every two or three days .

Discard or treat mycoplasma contaminated cells

In the case of mycoplasma contamination of cells which are really valuable and rare, it is suggested to treat them to eliminate mycoplasma infection. Otherwise, it is recommended to discard mycoplasma contaminated cells since they are considered as a source of contamination in the lab .