微米在細胞分離納米

廣泛的細胞分離技術(shù)本文中描述說明了高水平的興趣和活動在這一領(lǐng)域。描述的大小和基于密度的方法提供了一個潛力巨大的分離細胞亞種群的特定的標記不清楚或無法使用(例如防止細胞激活)。

基于吸引力的方法(熒光、磁、結(jié)合力和電泳)可以使用快速(~分鐘)和連續(xù)分離高特異性(~ 99%)。的所有方法，設(shè)備的設(shè)計是這樣的，他們可以在大規(guī)模并行的方式增加規(guī)模和吞吐量的前提下純度和效力。

此外，微流體分離系統(tǒng)可以很容易地整合與單細胞等設(shè)備，執(zhí)行下游分析裂解和蛋白質(zhì)組和基因組分析。先進水平的設(shè)計、制造和測量功能，我們預計，在未來幾年重點將從“概念驗證”的原型設(shè)備，很容易在經(jīng)濟生產(chǎn)和應(yīng)用，如即時臨床診斷方法、藥物發(fā)現(xiàn)，化學生物學代理檢測。

The broad spectrum of cell separation technologies described in this review illustrates the high level of interest and activity in this area. The described size- and density-based approaches offer a great potential for separation of cell subpopulations for which specific markers are not known or cannot be used (eg, to prevent cell activation). Affinity-based approaches (fluorescence-, magnetic-, adhesion-based, and electrophoretic) can be employed for fast (~minutes) and continuous separation with high specificity (~99%). For all of the approaches, the design of the devices is such that they can be operated in a massively parallel fashion to increase scale and throughput without compromising purity and efficacy.