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產(chǎn)品目錄
  • 細(xì)胞培養(yǎng)進(jìn)口血清
    進(jìn)口胎牛血清
    進(jìn)口新生牛血清
    進(jìn)口豬血清
    馬血清
  • 支原體檢測(cè)盒及標(biāo)準(zhǔn)品
    常規(guī)PCR檢測(cè)試劑盒
    熒光定量PCR檢測(cè)(qPCR法)
    支原體DNA提取
    靈敏度標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    特異性標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    PCR定量標(biāo)準(zhǔn)品(可用于方法驗(yàn)證)
  • 支原體祛除試劑
    細(xì)胞中支原體祛除
    環(huán)境支原體祛除
    水槽支原體祛除
  • 干細(xì)胞培養(yǎng)基
  • DNA/RNA污染祛除
    DNA/RNA污染祛除試劑
    DNA污染監(jiān)測(cè)
  • RNA病毒研究試劑
    RNA病毒檢測(cè)試劑盒
    病毒RNA提取
  • PCR儀器及配套產(chǎn)品
    DNA污染監(jiān)測(cè)祛除
    PCR/qPCR儀性能檢查
    PCR試劑
    PCR試劑盒
    PCR預(yù)混液(凍干粉)
    熱啟動(dòng)聚合酶MB Taq DNA
  • 微生物PCR檢測(cè)
    食品檢測(cè)類產(chǎn)品
    食品微生物檢測(cè)
    細(xì)菌PCR檢測(cè)

有效的抗支原體抗生素

2016-10-12 12:49

Autoclaving the contaminated cell cultures is the best way to get rid of the infections. In the case of valuable cells contaminated by mycoplasmas, autoclave cannot be helpful and an elimination method should be used without harming the eukaryotic cells. Besides the treatment of cell cultures, surfaces, cell culture media and supplements can also be treated by different methods including autoclaving, filtration, exposure to detergents, culture in the presence of 6-methylpurine deoxyriboside, passage through nude mice, and antibiotic treatment (77).

To eradicate mycoplasmas from FBS and trypsin, UV irradiation is more effective than the mentioned methods. In contrast to gamma irradiation, UV irradiation does not harm the serum components. UV irradiation can eliminate any potential contamination in trypsin or FBS (78).

A frequent check up is needed to verify a complete eradication of mycoplasmas. Sometimes mycoplasmas are suppressed and their titer decreases below detection levels of available assays. Thus, a sensitive mycoplasma testing is necessary to detect any reinfection in cell cultures. It is advised to recheck the cells in antibiotic-free medium after at least four to six subculture passages.

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