胎牛血清RNA干擾了細胞培養外源性RNA

Such enrichment would support an active and regulated mechanism of RNA secretion. Alternatively, exRNA may reflect the entire cellular transcriptome, a specific RNA class (e.g. miRNA), or one of the steps of RNA metabolism, and thus, provide a peripheral measure for monitoring the cellular transcriptome. Addressing this question is critical for the study of intercellular communication and development of RNA biomarkers. Cell culture systems employed in the studies of exRNA biogenesis and release, rely heavily on the fetal bovine serum (FBS), a commonly used reagent vital for the growth of numerous cell types. Although many reports characterized exRNA isolated from human and mouse serum and suggested serum RNA as the major source of disease biomarkers1, little attention has been paid to confounding effects of FBS-derived RNA on cell-culture based studies. Here, we characterize the RNA content of FBS and vesicle-depleted FBS (vdFBS), and demonstrate its interference with cell-derived exRNA isolated from conditioned media and, possibly, cellular RNA.